Abstract
In previous reports 1 , 2 the effect of blood serum on the toxicity of bile salts injected intraperitoneally into the white mouse and the hemolytic effect of these salts dissolved in normal saline for red blood cells were observed. It was found that hemolysis occurred in dilutions of 1:200 to 1:6400. This large variation in hemolytic range might have been due in part to exposure of the cells under diverse circumstances of collection and in part to the heterogeneous type of individual from whom they were collected.
The present report concerns the effects of blood serum on hemolysis by bile salts after the stock product had been purified as explained in a previous article. 1 Different concentrations of this purified salt were made up in normal saline and in fresh human serum (not older than 3 hours). A drop of fresh homologous blood was then added to the saline and serum dilutions and the results observed in 3 and 24 hours. The hydrogen ion concentrations of the solutions were computed but the variation was not sufficient to influence the results. The surface tension of the several solutions of like concentration of the salt was taken by the drop method and this variation was found in each instance to be so slight that at least by the present perfection of technique estimation of the small difference was impossible. The tubes in which the solutions were mixed were allowed to stand at room temperature. Approximately 24 bloods were tested.
When human serum was used as the vehicle and readings made in 3 hours, complete hemolysis occurred in the tubes containing 2.5% bile salts, partial hemolysis in tubes containing 1.25%, and no hemolysis in those containing 0.625%.
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