Abstract
In a preliminary report, one of us 1 described the formation of bone around a portion of a cyst lined by transitional epithelium resulting from transplantation of bladder mucosa to certain connective tissue areas (one of which is the sheath of the rectus abdominis muscle) in the dog. Similar transplantation in the rabbit is rarely followed by bone formation. The cysts contain a sero-sanguinous fluid in small amounts, rarely more than 4 cc., usually about 2 cc. when a circular area of bladder mucosa 2 cm. in diameter is transplanted.
It seemed of interest to determine the calcium and phosphorus content and the hydrogen ion concentration of this fluid and to compare these values with similar simultaneous determinations of the blood serum. In 3 rabbits in which bone did not occur around the transplant, determinations were made of the inorganic phosphates and hydrogen ion concentration of the cyst fluid and of the blood serum.
The fluid was aspirated through the skin with an 18 gauge needle and was drawn under oil. Calcium was determined by the Clark and Collip 2 modification of the Kramer and Tisdall 3 method in most instances. In 3 of the determinations the organic material was destroyed by heating with concentrated sulfuric acid before using the above method, with approximately the same results. Phosphorus was determined by the method of Fiske and Subbarow. 4 Carbon dioxide content of the serum and cyst fluid was determined by the manometric method of Van Slyke and Neill. 5 The bicolorimetric method suggested by Myers 6 and Hastings 7 was used for determining the hydrogen ion concentration.
The results are tabulated in Tables I and II.
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