Abstract
Amyloid has been produced experimentally in white mice by various workers, among whom are Kuczynski, 1 Letterer, 2 Smetana, 3 and Jaffé. 4 Nutrose (sodium caseinate) was injected daily. Smetana also used a cheese diet. Lucke and Markeley 5 have shown the difference of susceptibility to amyloid in rats and mice. They were unable to produce amyloid in rats by the injection of casein, but produced it in 75% of the mice.
The present study is upon the susceptibility of adrenalectomized rats to amyloid production. The rats were injected daily with 3 cc. of a 3% nutrose solution. The cheese diet used consisted of American cheese with a small amount of bread or whole wheat. When not fed on the cheese the rats were given a maintenance diet: 1/6 milk powder, 5/6 whole wheat, and salt in a quantity of 2% of the wheat.
A series of mice was also used. The mice were injected daily with 0.5 cc. of a 5% solution of nutrose. Other mice were fed on a diet of American cheese to which a small amount of bread was added. They were given water. The injected and control mice were fed on cracked corn, lettuce, carrots, and bread. They were not given water. Further facts on the 2 series are brought out in Tables I, II and III.
There are included in Table I, 4 unoperated rats that received injections and were fed on the cheese. In 4 rats (1, 5, 11, and 12) Congo red was injected into the heart and the rats killed one hour later. This was done to discover beginning amyloidosis. The liver, spleen, and kidney were examined in all rats, and other organs, in several.
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