Abstract
In a previous paper it has been shown by Bieter 1 that dilute solutions of mercuric chloride injected via the ureter in frog's kidneys, inhibit the reabsorption of water and phenolsulphonephthalein. Unpublished observations in this laboratory indicate that this action might also be shared by novasurol.
Attempting to obtain further information upon the fundamental action of novasurol as a diuretic, the effects of this drug in vitro and in vivo have been studied on the appearance of frog's plasma under the ultramicroscope. The technique of the ultramicroscope studies used has been that of Wright and Hirschfelder. 2 The novasurol in 0.1% concentration was made up in 0.75% NaCl. This gives 0.01 mgm. per 0.01 cc. To 1 cc. of frog's plasma diluted with 4 cc. of 0.75% NaCl solution was then added 0.04 mgm. of novasurol.
This amount of novasurol when added to a sample of frog's plasma, which was collected without the use of an anticoagulant, produced the following changes in particles as compared to those described in the preceding paper by Wright and Bieter. 3 The number of particles was decreased, their average size was increased, and their refractiveness, as measured with the photometer of Hirschfelder and Wright, 4 was increased.
When the same amount of novasurol was added to a sample of frog's plasma prepared as above, but where coagulation was prevented by the use of heparin (10 mgm. via lymph sac or 1 mgm. in 0.1 cc. saline placed in the tube used to collect the blood) the picture observed under the ultramicroscope was essentially the same.
The action of novasurol on citrated and oxalated plasmas has again progressed in the same general direction.
Get full access to this article
View all access options for this article.