Abstract
Loeb 1 , 2 and subsequently Hewlett, 2 Muraschew, 2 and Nolf, 2 have shown that tissue coagulins (thrombokinase of Morawitz, tissue fibrinogen of Wooldridge and Mills, thromboplastic substances of Schmidt and Howell, cytozym of Fuld, Spiro, and Bordet) are specific in different species of animals, the tissue coagulin of one species being relatively more efficient in causing coagulation of plasma from that species than from any other. Loeb, 3 and subsequently Loeb, Fleisher and Tuttle, 4 also found that when tissue extracts and blood sera are mixed together and incubated for varying periods of time, coagulation of plasma, added at the end of incubation, was delayed. In general, the longer the period of incubation the greater was the degree of inhibition of coagulation. Some evidence also pointed to a specific interaction between extracts and sera from homologous species and there was an indication that both accelerating and inhibiting effects were due to the interaction of substances specifically adapted to each other. The following experiments were carried out in an attempt to confirm and extend these observations.
Method. Constant amounts of kidney extracts and blood sera were incubated at 34° C. for periods varying from 0 to 80 minutes. At the end of these periods 1.0 cc. of heparized blood plasma was added and the time necessary for coagulation noted. Controls were made by substituting 0.9% NaCl solution for blood serum. Dog, goose, and chicken heparized plasma were used, and serum and kidney extracts were taken from the human, dog, rabbit, ox, sheep, goose, and chicken.
Results. Dog Serum with Various Kidney Extracts. Dog serum in combination with various kidney extracts produced inhibition of coagulation which became more pronounced with longer periods of incubation.
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