Abstract
Fourth generation of subcultures of B. typhosus, S. viridans, and certain other microorganisms grown in 10% horse serum (Ringer's solution) are agglutinated in dilutions of 1:20 to 1:160 by antihorse rabbit precipitin, controls cultures (nutrient broth) showing no agglutination.
With eighth to twelfth generation subcultures of the same microorganisms, definite agglutination takes place with 1:1000 anti-horse rabbit precipitin. The susceptibility of eighth to twelfth generation subcultures to precipitin-agglutination is not materially reduced by repeated washings with Ringer's solution. No agglutination of these subcultures takes place with any heterologous precipitin thus far tested (e. g., anti-egg rabbit precipitin).
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