Abstract
The monocytes of the blood were studied in normal rabbits as well as in those in which a monocytosis was produced. This was accomplished by two methods: one was the intravenous injection of lithium carmine, india ink (Higgins') and saccharated iron oxide in various combinations. The other method consisted of infecting the rabbits with B. monocytogenes recently isolated by Murray, Webb and Swan. 1 Some of these infected rabbits were also injected with lithium carmine while others were splenectomized. Two of the latter were slowly injected with one quarter of a cc. of Higgins'india ink in a branch of the portal vein so that the carbon would accumulate in the endothelial cells of the liver sinusoids.
The blood of the ear veins of all of the rabbits was studied by supravital, dry and wet smears. The animals were killed at various intervals after the appearance of the monocytosis. At necropsy, supravital, dry and wet smears were made of the various organs and vessels. Portions of all of the viscera and of the main vessels, including the thoracic duct, doubly ligated, were fixed in formalin-Zenker, embedded in celloidin, serially sectioned and stained with hematoxylin-eosin-azure II.
Get full access to this article
View all access options for this article.