Abstract
In continuation of the work reported in a previous note 1 the following results were obtained:
The precipitable substances extracted by dilute alcohol from various bacilli are of different sorts. While the substance derived from B. typhosus loses its activity by digestion with trypsin, the extracts of B. paratyphosus B and B. proteus X19 resist tryptic digestion like that of V. cholera. The substance extracted from B. paratyphosus B and B. proteus yielded reducing sugar after hydrolysis. The N content of the substance obtained from B. typhosus approximates that of proteins.
After treating B. typhosus several times with hot 75 per cent alcohol, an extract was prepared by heating with saline solution. This substance differed serologically from the above mentioned substance of typhoid bacilli, in that it was precipitated strongly by immune sera prepared with typhoid bacilli extracted with dilute alcohol, and gave weak reactions with immune sera prepared with dilute alcohol extracts. It is likewise digested by trypsin, and therefore both products apparently differ from that described by Douglas and Fleming. 2
After digesting with trypsiti, the yrecipitable substance extracted with dilute alcohol from B. paratyphosus B still reacted with B. paratyphosus B immune sera, but not with immune sera prepared with the dilute alcohol extract of B. typhosus or B. enteridis. This crude preparation of B. paratyphosus B produced strongly agglutinating and precipitating sera when injected into rabbits.
A second extract was obtained also from B. proteus HX19 with saline solution from the bacilli previously treated with dilute alcohol. In contrast to the dilute alcohol extract this preparation had a high N content and was digested by trypsin. Both the dilute alcohol and the saline extracts reacted with common HX19 and OX19 immune sera but gave practically no precipitations with typhoid and paratyphoid antisera.
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