Abstract
Since it has been proved that the transplanted stomach will secrete after a meal (Ivy and Farrel, 1 Lim, Loo and Liu 2 ), it has become necessary to obtain the exciting substance or substances from the blood, as the first step towards identification. We have, therefore, attempted to obtain the excitant (or excitants) by dialysing the circulating blood of dogs during digestion.
The animals were either narcotised with amytal, or given no anesthetic at all. The vessels were exposed (under novocaine in the latter case), and connected with a set of dialysing membranes (Necheles 3 ) immersed in warm Locke's solution. The blood was rendered incoagulable by recurring injections of heparin (Howell) or hirudin, or both. Circulation through the membranes was permitted for periods up to 5 hours, usually 2 hours, with the animal fed, 200 gr. meat and 100-200 cc. water, before or sometimes during the observation. The dialysate, concentrated or unconcentrated, was injected, subcutaneously, intravenously or intraperitoneally, into gastric pouch of dogs, whose secretory ability was determined by meal or histamine stimulation.
Three types of experiment were performed:
(1) Carotid artery and external jugular vein connected with membranes (carotid dialysis).
(2) Portal vein and external jugular vein connected with membranes (portal dialysis).
(3) Inferior vena cava, in thorax, and external jugular vein connected with membranes (caval dialysis),
The results in 14 satistactory experiments were as follows:
Carotid dialysis: Six experiments, with no anesthetic except novocainr locally, gave 2 dialysates doubtfully positive, 4 negative. The experiments, under amytal, gave both dialysates feebly positive (?) after a long latent period. 2 and 4 hours.
Portal dialysis: Five experiments (under amytal), gave 2 dialysates undoubtedly positive, latent period within first hour, 2 probably positive and 1 negative.
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