Studies of Biologic Properties of a Polysulfated Chondroitin,A73025 1

Abstract

Abstract

The chemical composition of a “heparin analog” (A73025) was studied and its biologic properties were compared with those of a heparin preparation from intestinal mucosa. A73025 was characterized as a polysulfated chondroitin with a molecular weight of about 10,000 M _r. Unlike heparin, A73025 showed poor anticoagulant activity in vitro by Stypven clotting time, partial thromboplastin time, and thrombin time assays. A73025, like heparin, although to a lesser extent, inhibited thrombin induced platelet aggregation, released lipoprotein lipase when injected into rabbits, and complexed with serum low and very low density lipoproteins.