Abstract
Summary
Treatment of herpes simplex virus type 2 (HSV-2)-infected human embryo lung cells with phosphonoacetic acid (PAA) at a concentration of 100 μg/ml did not inhibit the replication of HSV-2 over a 7-day period due to replication of low levels of PAA-resistant virus present in original virus stocks. Dose-response curves demonstrated that a minimum of 200 μg of PAA/ml was required to inhibit HSV-2 over this time interval. At twice the minimum concentration of PAA required, it was possible to induce a period during which infectious virus was un-detectable after reversal of drug treatment. This latent period was longer than that observed previously using cytosine arabinoside, and was extended by increasing the incubation temperature to 40° at the time of drug removal.
The authors wish to thank Dr. Stephen St. Jeor for continuous provision of HEL cells.
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