Abstract
Summary
Radioimmunoassays specific for rat serum albumin and rat α-fetoprotein have been described. These systems are capable of measuring the serum proteins in as little as one nanoliter of rat serum. Negligible cross reaction with other albumins and globulins was observed in sera from a variety of animal species. These radioimmunoassay systems have proved most useful in the quantitation of RSA and AFP in extracts from tissues where their mass and their affinities for estradiol could interfere with the estrogen receptor and hence compromise analyses of the binding events in estrogen action studies. Such interference would be amplified in the less mature rat, be influenced by the method of tissue preparation, and most likely occur in tissues characterized by femto and subfem-tomolar levels of estrogen receptor.
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