Abstract
Summary
Aggregation of human PRP by low concentrations of ADP, epinephrine or collagen, and of human WPS by collagen, was markedly potentiated if the PRP or WPS was premixed with small amounts of heated (56°, 30 min) normal plasma, thrombin-clotted plasma, hemophilic or von Willebrand plasma. Heated plasma samples by themselves did not induce platelet aggregation. The platelet aggregation potentiating activity of these samples resides with the heat-precipitated proteins. Neither serum nor a saline solution of fibrinogen was effective. However, the serum solution of fibrinogen was effective after heating. The inhibitory effect of aspirin was completely counteracted by small quantities of heated plasma in the reaction mixture. The effect of heat-precipitated plasma proteins appears to be on the platelet release mechanism, making it highly sensitive to minute amounts of aggregants.
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