Abstract
Summary
The 24-hr urine collected from streptozotocin-diabetic rats contained activities of acid phosphatase, alkaline phosphatase, N-acetyl-β-D-glucosaminidase, α-L-fu-cosidase, β-D-galactosidase, glutamate-oxal-oacetate transaminase, and α-D-mannosi-dase that were significantly greater than control values. Lactate dehydrogenase activity was similar in urines from diabetic and from control animals. Results were similar with rats injected with streptozotocin from 3 to 30 days prior to use. The levels of urinary acid hydrolase activities present in diabetic rats returned to control values upon treatment with insulin and became elevated above control values once again upon cessation of insulin treatment. The levels of enzymatic activity measured in diabetic rat urine, except for LDH, were directly proportional to the volume of urine produced. However, normal animals with induced polyuria did not produce urines with enzymatic activities above control values.
This study was supported by USPHS Grant 5685-12, the California Metabolic Research Foundation, and the Kroc Foundation. M. D. K. was a recipient of a summer fellowship from the California Foundation for Biochemical Research. We thank Dr. Arne N. Wick for his encouragement and for use of his laboratory facilities.
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