Abstract
Summary
A monolayer culture of fibro-blast-like cells was initiated and established from a trypsin digest of Omaka fish larvae (Caranx mate). The cell culture has been subcultured 105 times over a period of 30 mo and appears to be contact-inhibited.
The Omaka cells grew optimally at 27° in Eagle's basal medium plus 10% fetal calf serum but failed to grow at 16 and 37°. At the lower temperature the cells remained viable and began to divide when the temperature was raised to 27°. Unlike certain marine fish cell lines the Omaka cell did not require a higher osmolarity medium for optimal growth. Karyological analysis at the 43rd passage revealed a chromosomal modal number of 50 consisting of 7 submetacentric pairs and 18 acrocentric pairs of chromosomes. Virological studies indicated that Omaka cells supported the replication of poikilothermic viruses such as, FV-3 and IPN viruses, but not the mammalian viruses examined. Attempts to induce interferon by the synthetic polynucleotide poly I:C were unsuccessful.
This investigation was supported in part by Project No. R/04-01 Sea Grant Programs; National Oceanic and Atmospheric Administration, U. S. Department of Commerce.
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