Aminoisobutyric Acid Transport in Primary Cultures of Normal Adult Rat Hepatocytes 1

Abstract

Summary

In contrast to suspensions of freshly isolated hepatic parenchymal cells (HPC), short-term monolayer cultures of HPC displayed properties of active transport for the amino acid analog aminoisobutyric acid (AIB). The uptake of AIB was inhibited by KCN and iodoacetate, failed to occur at 4°, and was stimulated by glucagon. The apparent K_m for AIB uptake by cultured HPC was approximately 19 mM. Glucagon did not alter the apparent K_m but did increase V.

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