Abstract
Summary
A method for specific radioactivity analysis of alanine in blood is described. This analysis involves the separation of alanine by an ion exchange column technique and its subsequent isolation as a gravimetrically assay able sample (acetaldehyde-dimedon). Mass contamination of the acetaldehyde-dimedon from valine, which elutes from the ion exchange column close to alanine, is demonstrated to be negligible. Activity contamination of the acetaldehydedimedon from lactate and glucose, two significant products of alanine metabolism, is likewise demonstrated to be negligible. Sample counting rates have been demonstrated to be significantly above background (X 3) 2 hr following a single injection of 25 μCi l-[14C] alanine to a human subject.
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