Abstract
Summary
The XC cell line was developed from tumor cells induced in newborn Wistar rats by the Prague strain of Rous sarcoma virus (RSV). Attempts to propagate herpes simplex virus type 1 (HSV-1) or herpes simplex virus type 2 (HSV-2) in these cells proved futile. However, both virus types were able to replicate to high levels in control nontransformed Wistar rat embryo (WRE) cells.
Examination of this abortive infection revealed that both HSV-1 and HSV-2 were able to attach to the XC cells but were unable to induce virus protein, DNA, or specific thymidine kinase. Furthermore, while cell DNA synthesis was severely depressed in receptive WRE cells, no such effect was observed in resistant XC cells. These studies suggest that an early event, prior to viral DNA synthesis, is blocked in the replicative cycle of HSV.
This study was conducted in part under support funds from the U.S. Public Health Service Biomedical Grant No. 5 S05 R RO7082-07 and the Pennsylvania Agriculture Experiment Station Project No. 1992. Authorized for publication as paper No. 4439 in the journal series of the Pennsylvania Agriculture Experiment Station.
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