Effect of Synthetic Thyrotropin-Releasing Hormone on Glucose Oxidation and Phospholipogenesis in Porcine Anterior Pituitary Slices in the Presence or Absence of Thyroid Hormone

Abstract

Summary

Synthetic TRH stimulated the oxidation of ¹⁴C-1 -glucose and ¹⁴C-6-glucose to ¹⁴CO₂ in porcine anterior pituitary slices. This effect of TRH on the oxidation of ¹⁴C-6-glucose was greater than that of ¹⁴C-1-glucose. Thyroxine blocked the increase of glucose oxidation produced by TRH, but failed to do so in the presence of actinomycin D. Synthetic TRH also stimulated ³²P incorporation into phospholipids, the magnitude of stimulation being related to the dose of TRH used. DBcAMP also stimulated phos-pholipogenesis by the anterior pituitary. TRH stimulation of pituitary phospholipogenesis was not inhibited by triiodothyronine.