Abstract
Summary
Effects of various incubation temperatures (30, 37 and 40°) on type 1 and type 2 strains of Herpesvirus hominis (HVH) were studied in primary cultures of rabbit kidney cells. Eight strains of type 1 and 7 strains of type 2 HVH were used. These strains included both recent isolates and laboratory strains with long histories of passage in various tissues.
Both type I and type 2 HVH strains, regardless of passage history, grew slower at 30 and 40° than at 37°, but this retardation of growth was more pronounced in cultures infected with type 2 strains of HVH at 40°. The production of cytopathic effects (CPE) and the growth of type 2 HVH in rabbit kidney cells were almost completely suppressed as long as the infected cells were maintained at 40°. Upon transferring the cells to 37°, however, CPE along with rising infectivity titers of the virus were noted. Further spread of CPE produced by type 2 HVH following incubation at 37° was successfully blocked by incubating the cells at 40°.
Fig. 2. Minimum infectious doses of type 2 Herpesvirus hominis following incubation at 37°, then at 40°. (1) at 37° for the entire period; (2) at 37° for 1 day, then at 40° thereafter; (3) at 40° for the entire period. The minimum infectious dose is defined in Fig. 1.
This work was supported by Grant EY 00310 from the National Eye Institute.
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