Abstract
Summary
Rabbits were hyperimmunized with bovine myelin basic protein (BP) or its acetylated derivative (AcBP). The sera were studied by means of passive hemagglutination with formalinized, tannic acid-treated sheep erythrocytes coated with BP or AcBP. Formalin treatment eliminates the nonspecific clumping of BP-coated sheep cells, which we previously encountered. Sera were also analyzed by immunodiffusion in agarose. The rabbits immunized with BP produced antibodies which reacted equally well with BP and AcBP. However, the rabbits immunized with AcBP formed antibodies which were apparently directed against the acetyl group, in addition to anti-BP antibodies.
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