Abstract
Summary
A DEAE-cellulose column chromatographic technique was used for separation of TCII, MPPB and TCI in 1 ml of human serum. The percentage of vitamin bound to each binder remained practically constant at levels of added vitamin varying from 250 to 5000 pg 57Co B12/ml of serum.
The percentage 57Co B12 bound to MPPB and TCI in chronic myelocytic leukemia serum was significantly higher than in normal serum.
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