Abstract
Summary
The induction of delayed hyper-sensitivity in mice to purified acidic (anionic) proteins by injecting these subcutaneously in incomplete Freund adjuvant appears to require the aid of phosphate ions in the aqueous phase of this adjuvant. Arthus hypersensitivity to these antigens is readily induced by such injections whether phosphate ions are present or not. Phosphate may function by affecting host cell-antigen associations in the earliest stages of induction. These experiments suggest that a more detailed investigation of the functions of the aqueous phase of Freund adjuvants is needed, and also that for at least some models of delayed hypersensitivity incomplete Freund adjuvant can be used in place of complete Freund adjuvant if phosphate ions are present.
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