Abstract
Summary
Different populations of guinea pig lymphoid cells demonstrate marked heterogeneity in their ability to elaborate an in vitro chemical mediator of cellular immunity, migration inhibition factor. A concentration of 2.5% lymph node cells obtained from animals demonstrating cutaneous reactivity to tuberculin protein effectively inhibited the migration of peritoneal exudate cells from normal donors. Thymic and bone marrow cells were relatively ineffective in this assay; no significant reduction in the area of migration was observed with cell concentrations as high as 20%. The presence of a second but numerically small population of immunologically active cells in both these central lymphoid organs was suggested by the inhibition of migration in assays using 25% cells from sensitized donors. Results of these in vitro assays were further confirmed by in vivo cell transfer experiments.
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