Abstract
The paper is a report of an attempt to estimate the circulation by the difference in the oxygen content of arterial and venous blood.
1. Arterial Blood. — Being unable to draw samples from an artery, we take the venous blood, saturate it with air, and determine either its oxygen content or its hemoglobin content, which is proportional to the oxygen capacity. The oxygen content is determined by Van Slyke's method, previously described in the Proceedings of this Society. The hemoglobin is determined by a colorimetric method of Dr. W. W. Palmer.*
2. Venous Blood. — Ten c.c. of blood, drawn from the arm vein without any stasis, is deposited together with a little oxalate below mineral oil. A sample of 2 c.c. is transferred to the chamber of the Van Slyke apparatus without exposure to air, and the chemically bound oxygen determined.
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