Abstract
Summary
A method was developed for preparing antibody to Fischer-344 rat transplantation antigen, using as an antibody source serum from Buffalo rats immunized by repeated transplants of a carcinogen-induced Fischer tumor. Immune y-globulin was labeled with 125I and radioactive antibody isolated by twice repeated adsorption onto Fischer red blood cells and elution from stroma prepared from these cells. In vitro such antibody bound specifically to Fischer red blood cells. In vivo this antibody localized preferentially in the Fischer tumor grown in immunologically suppressed Buffalo rats.
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