Abstract
Summary
The microepidemic in primary human amnion cells infected with herpes simplex virus was studied by tritiated thymidine (Tdr3H) autoradiography, time-lapse cinematography, and fluorescent antibody techniques. The plaque is formed by retraction of the cell sheet and the sequence of infection is preserved in rings of cells around the plaque. The progress of the plaque may be followed in irradiated cultures in which essentially all of the DNA synthesis is virus induced. The cell-to-cell transmission as opposed to the cell generation time was 8-10 hr as determined by three different techniques. The presence of labeled DNA and specific HSV protein in the intercellular bridges indicates the means by which the virus spreads in a culture.
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