Abstract
Summary
Bovine LH was labeled with 125I by chloramine T oxidation and gel filtration. Immunological characterization of iodinated LH, using either gel filtration on Sephadex G-200 or double antibody precipitation to separate free and antibody-bound 125I-LH, indicated that 71 and 64%, respectively, of total radioactivity was reactable with antibody to bovine LH.
A dose of 125I-LH within the physiological range (51 ng/100 g of body wt) was administered intravenously to immature female rats. Serum samples, ovaries, adrenals, thyroids, and portions of liver were taken for radioassay at times ranging from 5 min to 12 hr following injection. Disappearance of radioactivity from blood approximated a hyperbolic function with a half-life of 19.6 min. Radioactivity in liver at its 15-min maximum represented 43% of the total injected dose per whole organ. Radioactivity in ovary and adrenal appeared at similar low levels (0.05-0.07% of injected dose per pair of glands). Disappearance curves from the two organs, however, were significantly different, with radioactivity in ovary, but not in adrenal, being retained up to 30 min following injection.
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