Selected Enzyme Activities and Isoenzyme Patterns of Virus-Infected Cell Cultures 1

Abstract

Summary

Specific enzyme activities and isoenzyme patterns were determined in cell-free extracts from uninfected and virus-infected mammalian cultures. Samples were harvested at intervals prior to the appearance of morphologic changes in the cells, Two enzymes of the Embden-Meyerhof pathway, 2 of the tricarboxylic acid cycle, 1 in the hexose monophosphate shunt, and 4 hydrolytic enzymes were tested. Enzyme assays revealed differences in specific activities from infected cells as compared to controls. The main differences occurred during the early stages of contact between virus and cells and at a late time period when viral particles were accumulating in the cells. The malate dehydrogenase isoenzyme patterns from cultures infected with pseudorabies virus and harvested just prior to the appearance of morphologic changes had a different pattern than those of comparable uninfected cultures. The difference involved a predominance of the anodal band with some deletion of the bands that remained closer to the origin.