Abstract
Summary
Blast cell transformation in vitro of lymphocytes obtained from the peripheral blood of guinea pigs after sensitization to mycobacterial antigen was shown to increase after PPD stimulation. However, the cellular activity of lymphocytes was influenced by recent sensitization or immunization of the animals. The blastogenic response of guinea pig lymphocytes to the mitogen PHA was decreased following both the initial tuberculin sensitization and skin testing with PPD. Lymphocytes from tuberculin-sensitized animals demonstrated a fall in responsiveness to in vitro PPD stimulation after the animals were skin tested.
Furthermore, this study shows that in vitro NDV infection of sensitized lymphocytes prevents the cells from undergoing PPD-induced transformation. This confirms earlier work on viral-induced lymphocyte unresponsiveness and establishes the guinea pig as an animal model for investigating the virus-lymphocyte relationship.
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