Abstract
Summary
A method for isolation of mammary gland cell nuclei was described. DNA-dependent RNA polymerase activity in isolated mammary gland nuclei was measured in rats fed a single dose of DMBA. In female rats, there was a significant inhibition of Mn2+-(NH4)2SO4-activated RNA polymerase activity within 24 hr after the feeding of DMBA. Inhibition continued for 2 days, but thereafter, the enzyme activity rose significantly above the control value. In contrast, RNA polymerase activity was not depressed in male rats treated with DMBA.
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