Abstract
Summary
Pichinde virus, a virus capable of producing a chronic infection, was found to replicate in Vero cells and to form plaques in these cells. The virus had a latent period of approximately 8 hr and maximum titers were reached by 24 to 36 hr after infection. Adsorption of the virus was enhanced in the presence of 10 to 100 μg/ml of DEAE-dextran. Pichinde virus was found to be relatively thermostable, stable between pH 5.5 to 9.0 and sensitive to ethyl ether. Virus replication was not effected by the DNA inhibitors, IudR and ara-C; however, virus replication was inhibited by actinomycin D at concentrations of 1 and 4 μg/ml. The virus was stable in sucrose and cesium chloride and densities of 1.18 and 1.19 to 1.20 g/cm3, respectively, were obtained in gradients made of these two substances.
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