Abstract
Summary
A bioassay for mycotic sensitins has been developed which yields a potency ratio with confidence limits for new lots of sensitin relative to a standard lot. The standard and test sensitins are injected intrader-mally into homologously sensitized guinea pigs and the diameters of the resulting skin reactions are submitted to a Control Data 3200 computor programmed to perform an analysis of variance and regression analysis. The potency ratio obtained by this procedure can be used as a dilution factor for the standardization of new lots of sensitin.
The practicality and reproducibility of the bioassay as a standardization procedure was demonstrated by assays of coccidioidin from 4 strains of Coccidioides immitis. Coccidioidin Gasket was found to be equal in potency to standard coccidioidin while coccidioidin Rystead was found to be less potent. Coccidioidin from strains F842 and Ruddy were unsuitable for standardization as skin test antigens by this procedure.
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