Abstract
Summary
A conjugate subjected to diethyl-aminoethyl-cellulose chromatography and gradient elution to eliminate both underconjugated and overconjugated antibody molecules has been used to localize specifically Chikungunya, Mayaro, and Semliki Forest viruses in HeLa cells. Immunofluorescence was present in the cytoplasm only and was diffuse in nature; nonspecific staining was negligible. The three viruses, closely related members of arbovirus group A, were readily distinguishable by the indirect fluorescent antibody (FA) technique. The FA titers of homologous systems were reproducible and uniformly higher than the FA titers obtained in heterologous systems.
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