Abstract
Summary
Protein synthesis in FMDV-infected BHK cells was inhibited in cells incubated at 25 to 41°. The metabolism of infected cells was always highest at 33°. Infected cells incubated at 30 to 41° produced the same high levels of virus, but in times ranging from 12 to 4 hr, respectively. Virus-induced RNA polymerase activity was always highest at 37°, being about twice as great as that formed at any other temperature. The cell-free polymerase system was active from 25 to 50° and showed maximal activity after 60-min incubation at 37°. Sucrose gradient profiles of the products formed under all the conditions employed did not reveal any qualitative differences.
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