Abstract
Summary
The concentration of radioactivity in different tissues of Yorkshire pigs 45 min after an iv injection of 3H-hydrocortisone was determined. Also, chromatographic separation of the tissue supernatant fractions was conducted. Only the liver was capable of concentrating radioactivity from the blood. Radioactivity in the brain, thymus, and heart was less than in the plasma, while activity in the spleen, skeletal muscle, testes, and pituitary was the same as plasma. Molecular-sieve chromatography of the supernatant fractions from the liver, spleen, and thymus on the Sephadex G-100 indicated part of the radioactivity was present in the macromolecular fractions excluded from the gel. It is possible that a relationship between the intracellular binding of the radioactive steroid and its physiological actions may exist.
The authors express sincere appreciation to Jennifer Couts for technical assistance during the course of this investigation.
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