Abstract
Summary
Neoplastic transformation of rat embryo cells in vitro by an osteosarcoma (FBJ) virus is reported. Foci of transformed cells consisted largely of spindle-shaped cells which stained vividly with acridine orange. The transformed cells produced virus and complement-fixing (CF) antigen characteristic of the murine leukemia-sarcoma virus complex. Tumors were produced when transformed cells were injected into newborn mice and rats. The mouse tumors had the histologic pattern of osteosarcoma, which was transmissible in cell-free passages in newborn mice. Cell-free extracts of the mouse tumors also produced transformed foci in vitro. The rat rumors were undifferentiated sarcomas, also yielding virus and CF antigens.
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