Abstract
Summary
Soluble and insoluble aggregates are major inactive radiation products in vacuo from crystalline lysozyme, ribonuclease, and trypsin. Soluble aggregates can be cleanly separated from active monomer materials by gel filtration on P-100 columns. This separation also permits quantitation of aggregate formation. Such products increase with increasing dose. If H2S is present during radiation, aggregate yield is decreased in ribonuclease and increased in lysozyme and trypsin. In the case of the latter two enzymes aggregates produced in the presence of H2S retain significant activity levels. These results indicate the free radical scavenger, H2S, promotes aggregate formation from active monomers in which free radicals have been produced by irradiation.
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