Abstract
Summary
Human lymphocytes were cultured in diffusion chambers implanted into the peritoneal cavities of rats. In cultures treated with PHA, the pattern of morphologic transformation and initiation of DNA synthesis paralleled closely similar studies made in vitro. Immunologic stimulation of the human cells by the heterologous host was presumably minimal or absent since control cultures (without PHA) showed no significant degree of blastogenesis when compared with PHA-treated cultures. Minimum DNA synthesis time determined for the PHA-stimulated lymphocytes was 9–10 hr. Values found for the duration of Tc (16–18 hr) and minimum TG2 (2 hr) were shorter than those reported for similar studies in vitro. The in vivo culture method using Millipore chambers appears to offer a more physiologic method for studying lymphocytes.
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