Abstract
The observations which are contained in this summary are based on the microchemical demonstration of potassium in the kidney cells of thirty-four dogs. The animals have varied in age from four months to something over ten years. Four of the animals may be grouped as “normal animals.” They did not receive any nephrotoxic substance and neither were they subjected to the action of an anesthetic. After a period of three days of observation these animals were killed by shooting.
The remaining thirty animals were rendered nephropathic by uranium nitrate in the dose of 4 mg. or 6.7 mg. per kilogram. They were anesthetized by either Gréhant's anesthetic in 60 per cent. strength, or by morphine-ether.
At the termination of the experiment small pieces of kidney tissue were removed, and frozen sections not over 20 micra in thickness were made. The sections were treated at once with Erdmann's 2 reagent as modified by Macallum 3 and used by him in his studies “On the Distribution of Potassium in Animal and Vegetable Cells.”
The reagent which consists in a solution of the hexanitrite of cobalt and sodium serves as a complete precipitant of potassium from its solutions, in the form of an orange-yellow precipitate of the triple salt. If the salt is present in minute quantities the crystalline form is absent. To render the detection of small quantities of the salt possible, Macallum 4 used ammonium sulphide to react with the cobalt of the salt and form the black sulphide of cobalt which is easily detected. This suggestion of Macallum's has been employed in the demonstration of potassium in all of the sections.
The results which have been obtained are as follows.
1. The epithelial cells of the normal dog kidney show only traces of potassium. The potassium is most marked in the loops of Henle and is fairly evenly distributed throughout the cytoplasm of the cells. It has never been demonstrated within the nucleus of the normal cell.
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