Abstract
Summary
The data presented indicates that the site of priming affects the local inflammatory cellular response to subsequent challenging injections. Animals sensitized with one or two subcutaneous injections showed a marked and persistent eosinophilia following a challenging intraperitoneal injection. On the other hand, animals sensitized by 1 or 2 intraperitoneal injections had consistently fewer eosinophils and they routinely peaked on day 1. Animals receiving 3 injections in which the site of the second injection differed from that of the primary had intermediate numbers of eosinophils. Neutrophils and mononu-clear cells showed no consistent differences in their response pattern. Repeated i.p. injections produced higher antitoxin titers than any other combination tested. There appears to be an inverse correlation between the circulating antitoxin at the time of challenge and the subsequent eosinophil responses. Animals with high antitoxin titers have fewer eosinophils in the inflammatory exudate. These results do not support the concept that the local eosinophilia is due to antigen-antibody reactions per se, but suggest instead that the local eosinophilia may be inhibited by the presence of antibody at the time of challenge.
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