Effect of Connective Tissue and Collagen on Platelet Lactate Production

Abstract

Summary

Although connective tissue and collagen induced the formation of huge platelet clumps, they had different effects on lactate production. With collagen, platelet lactate production was linear for 60 min, as compared with 45 min for control platelet suspensions. The rate of lactate production increased during actual aggregation and was similar to that of the control thereafter. Collagenase-treated collagen behaved like the control samples. In contrast to collagen, CT caused an arrest of lactate production within 15 min. Prior to this time, more lactate was produced than in the controls. Collagenase-treated CT also arrested lactate production within 15 min but caused no aggregation. After the addition of hyaluronidase-treated CT or CT with added Mg²⁺, however, aggregation occurred, and lactate production proceeded linearly for 60 min, similar to the rate for control or collagen-treated platelets. These findings appear to show that the arrest of lactate production by CT was due to the binding of divalent cations by acid mucopolysaccharides.