Abstract
Summary
A reproducible and simple chromatographic method with DEAE-Sephadex was used for fractionation of GOT isoenzymes. In the sera of health individuals three distinct isoenzymes were found. In patients with liver diseases, the SGOT isoenzyme distribution was distorted. Two isoenzymes, (A1 and B), were substantially increased, whereas the third one (A2) remained the same. More striking changes were noticed in acute viral hepatitis. Isoenzyme A2 was not detectable, and the isoenzyme pattern was similar to that in liver homogenates. A possible explanation of this altered isoenzyme distribution was discussed. The change of isoenzymes in the sera reflect more precisely the extent of the hepatocellular damage, rather than the total GOT activity.
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