Effect of Hypothalamic Extracts on Protein Synthesis in Rat Anterior Pituitary Tissue ∗

Abstract

Summary

Rat anterior pituitary glands cultured for periods up to four hours incorporate amino acid-¹⁴C mixture or glutamic acid-¹⁴C into pituitary protein in linear fashion. Addition of hypothalamic extracts to the culture system results in an increased incorporation and release of protein radioactivity as compared to glands incubated with cerebral cortex extracts. The stimulatory effect can be demonstrated at the level of the pituitary polyribosomes.