Abstract
Summary
Under certain experimental conditions, a specific antigenic component of pregnant mare's serum gonadotropin (PMSG) reacted with a hyperimmune antiserum to ovine luteinizing hormone (LH) to form an early-appearing heavy precipitin line in agar gel double diffusion preparations. It was demonstrated that it interfered with the free diffusion of some unrelated antibodies in a multiple precipitating antigen/antibody system. In addition, removal of this line by treatment of PMSG with urea unmasked a well-defined line identified in another study as a contaminant of follicle-stimulating hormone (FSH). Failure of the latter line to appear under similar conditions when untreated PMSG was used was ascribed to the interference with diffusion of the FSH contaminant through the strong mechanical barrier formed by the specific component of PMSG.
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