Abstract
Summary
A spectrophotometric method using DTNB for the estimation of nonprotein -SH in the presence of protein -SH groups in rat brain homogenates is described. Details of the optimum conditions for the color development which were found to be dependent on the reaction time and pH are also given. Estimates of nonprotein -SH and total -SH in rat brain reported by a number of investigators using various methods are in good quantitative agreement with those obtained in this study. Thus, findings led us to conclude that the relative specificity, rapidity, and accuracy of this chemical method are comparable with the existent methods requiring specialized instruments or techniques. This spectrophotometric method has a further advantage of not requiring measurement in the region between 250 and 280 mμ where interference by ultraviolet-absorbing substances is often a complication.
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