Abstract
Summary
A high level of immunity of long duration was produced in CF1 white Swiss mice against Sarcoma 180 and Ehrlich carcinoma by 3 weekly injections of homologous tumor cells killed by ultraviolet irradiation, at 2537 Å. Intraperitoneal route of immunization afforded more protection than the subcutaneous route. Significant immunity to challenge with living Sarcoma 180 cells was still present eight months after immunization. Sarcoma 180-immunized mice were also cross immune to Ehrlich carcinoma. Passive transfer of serum from Sarcoma 180-immunized mice did not protect nonimmunized mice. Neither precipitins nor agglutinins could be demonstrated in the sera of the immunized mice against homologous intact or disrupted tumor cells or cell supernates. The loss of immunogenicity of tumor cell vaccine upon cell disruption and the absence of humoral antibodies and passive transfer leads to the conclusion that immunity produced by irradiated, killed tumor cell vaccine is primarily cell mediated.
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