Activation of Trypsinogen by Plasmin. ∗

Abstract

Summary

Trypsinogen was activated by streptokinase-activated plasmin; and the optimal concentration of CaCl₂ was 0.02 M. By avoiding higher concentrations of calcium, and by using a dilute solution of trypsinogen which contained little trypsin, it was possible to limit interference from autocatalysis. Under these conditions, the rate of activation depended on the amount of plasmin. An incubated mixture of urokinase and plasminogen was able to activate trypsinogen, whereas neither urokinase nor plasminogen did so alone.