Abstract
Summary
Freshly isolated frog gastric mucosa secretes hydrochloric acid, under appropriate conditions, in the absence of added his-tamine. However, if the mucosa is incubated with histamine-free media, the acid secretory rate decreases to a very low rate after approximately 16 hours, the p.d. increases, and the short-circuit current declines. Addition of 10-4 M histamine to the nutrient medium bathing the serosal side restores acid secretion to about 80% of the initial rate; there is little change in p.d. and an increase in short-circuit current is observed. No difference exists in the ATPase activity of the resting and hista-mine-stimulated mucosa; the ATP level of the stimulated mucosa, on the other hand, is slightly greater than that of the resting mucosa. The procedure of preincubation of the mucosa in histamine-free media has made it possible for the first time reproducibly to obtain resting mucosae which can be stimulated by added histamine.
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