Abstract
Summary
A histochemical method was modified to demonstrate ATP hydrolysis by nuclei isolated from embryonic myocardium. Under a variety of experimental conditions, the results are in agreement with previous histochemical studies using cryostat sections and with enzymatic studies using isolated nuclei. The data give further support to the localization of ATP phosphohydrolase activity both at the nuclear surface and in the nucleo-plasm, under conditions which eliminate possible diffusion artifacts resulting from enzymatic activity by other cell components and non-enzymatic hydrolysis of ATP by Pb++.
The author is indebted to Mrs. A. P. Breland and Mrs. A. T. Klein for technical assistance and to the University Medical Center Illustration Department for photography.
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